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KMID : 0380619800120010045
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Korean Journal of Food Science and Technology
1980 Volume.12 No. 1 p.45 ~ p.52
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Hydrolysis of lactose in Milk by Microencapsulated β - Galactosidase
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변시명/Byun, Si Myung
백옥련/로사위/Baik, Ok Ryun/Uy, Rosa
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Abstract
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The nylon (poly 6, 10) microcapsules containing β-galactosidase were obtained by the interfacial polymerization of 1, 6-diaminohexane and sebacoyl chloride with β-galactosidase from Escherichia coli. They were generally spherical and had a mean diameter of 80 μ with 45 % of the activity recovery. In particular, there was no transport hamper of lactose through the membrane of microcapsules.
The characteristics of the microencapsulated enzyme were similar to those of soluble enzyme optimal pHs, 7.0∼7.2 for the soluble and 7.3∼7.5 for the microencapsulated ; optimal temperatures, 50℃ for both ; apparent K_m, 3.33×10^(-4) (on ONPG), 2.86×10^(-3) M(on lactose) for the soluble and 5.28×10^(-4) (on ONPG), 4.25×10^(-3) M (on lactose) for the microencapsulated ; activation energies, 8.94 for the soluble and 9.78 Kcal/mole for the microencapsulated enzyme.
Using this microencapsulated β-galactosidase, hydrolyses of lactose and milk lactose were carried out and 80 % of 5 % lactose solution and 70 % of lactose in skim milk were hydrolyzed in 40 hr at 27℃. The reusability and operational stability showed that the remaining activity was 50 % of the original activity after 5 runs and 120 hr of total operating time at 27℃.
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